Immobilization of IMP-1 metallo-beta-lactamase on Fe3O4@SiO2 as nanobiocatalyst for degradation of beta-lactam antibiotics in wastewater

Abstract Residues of antibiotics in water resources and wastewater have been significant environmental public health problems. The current study developed a high-efficiency enzymatic nanobiocatalyst for the degradation beta-lactam antibiotics. For this purpose, metallo-beta-lactamase IMP-1 was obtained by cloned blaIMP gene overexpressed Escherichia coli. 2.6 mg purified enzyme used immobilization on 100 modified Fe3O4 @ SiO2 magnetic nanoparticles. Immobilized showed similar storage stability to free enzyme. optimum temperatures activity pH immobilized enzymes were 70 °C 60 °C, 7.5 6.5, respectively. In addition, after 15 reaction cycles, 80 percent retained, according reusability analysis enzymes, highest catalytic observed penicillin G cephalexin, whereas Vmax/Km value ceftriaxone 3-fold (free enzyme) 10-fold (immobilized lower than G. Also, results that nanoparticles has an excellent ability remove from aqueous solutions. Thus, i could be appropriate choice removing pharmaceutical industry wastewater.